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The autofocus system on the a6400 inherits many technologies from Sony's line-up of full-frame cameras, including the a9, a7R III, and a7 III models. The a6400 features 425 phase-detection AF points and 425 contrast-detection AF points that are placed densely over the entire image area, covering approximately 84% of the image area. This high-speed, high-performance tracking AF system is paired with a BIONZ X image processing engine that together allow the camera to acquire focus in as little as 0.02 seconds and maintain subject lock effectively, ensuring even the fastest moving subjects can be tracked and captured with ease.

The a6400 introduces advanced "Real-time Eye AF," the latest version of Sony's Eye AF technology. This capability employs artificial intelligence-based object recognition to detect and process eye data in real time, resulting in improved accuracy, speed, and tracking performance of Eye AF. In all autofocus modes, the camera now automatically detects the eyes of the subject and activates Eye AF with a half press of the shutter button, and when in AF-C or AF-A mode, the preferred eye (left or right) of your subject can be selected as the focus point. Choices include Auto / Right Eye / Left Eye, and a Switch Right / Left Eye function is able to be assigned to a custom function as well. This technology frees the photographer to focus solely on composition with trust that focus will be tack sharp on the subject's eye. Eye AF support for animals will be added in summer 2019 via a system software update.

Also debuting on the a6400 is Sony's "Real-time Tracking." This mode utilizes Sony's algorithm that includes artificial intelligence (AI) based object recognition and processes color, subject distance (depth), and pattern (brightness) as spatial information to ensure that all subjects can be captured with accuracy. Additionally, when photographing or videographing humans or animals, face and eye position information is recognized by AI and the subject's eye is monitored in real time with high tracking precision. This can be activated by a simple half press of the shutter button or can be assigned to a custom function

In terms of overall shooting speeds, the camera can shoot at up to 11 fps with full AF/AE tracking while utilizing the mechanical shutter, and up to 8 fps with full AF/AE tracking while silent shooting. It can shoot at each of these speeds for up to 116 frames JPEG Standard / 46 frames raw compressed, greatly increasing the chances of capturing the moment.

Sony's a6400 is equipped with a 24.2MP APS-C image sensor that is paired with an upgraded BIONZ X processor to deliver improved image quality and color reproduction in all types of shooting conditions. Standard ISO ranges up to ISO 32,000 for both stills and movies and is expandable up to ISO 102,400 for still images, with good noise reduction at medium and high sensitivities.

The camera also inherits many of the image processing algorithms from Sony's full-frame cameras, suppressing noise while preserving resolution and improving texture depiction.

The versatile a6400 is a solid video camera, offering internal 4K UHD (3840 x 2160) movie recording with full pixel readout and no pixel binning to collect about 2.4x the amount of data required for 4K movies, and then oversamples it to produce high quality 4K footage with detail and depth. Focusing during movie shooting is fast and stable thanks to upgraded Fast Hybrid AF technology, which keeps the subject in constant smooth focus, even if an object crosses in front of the camera. This advanced AF plus touch focus functionality makes it a suitable camera choice for many vloggers and video creators that are regularly creating and uploading content online.

For time-lapse movie creation, the camera features built-in interval recording that can be set anywhere between 1 and 60 seconds, with a total number of shots from 1 to 9999. AE tracking sensitivity can be adjusted to "High", "Mid," or "Low" during interval shooting, allowing for reduced changes in exposure over the shooting interval.

Additionally, for the first time in a Sony APS-C mirrorless camera, this model includes an HLG (Hybrid Log-Gamma) picture profile, which supports an Instant HDR workflow, allowing HDR (HLG) compatible TV's to playback beautiful, true-to-life 4K HDR imagery. Further, both S-Log2 and S-Log3 are available for increased color grading flexibility, as well as Zebra functionality, Gamma Display assist, and proxy recording. The camera can also record Full HD at 120 fps at up to 100 Mb/s, allowing footage to be reviewed and eventually edited into 4x or 5x slow-motion video files in Full HD resolution with AF tracking.